Question 1

Two small plasmids undergoing recombination will look like a figure 8. If they are each cut once at a unique restriction site, they will form a Chi structure. Which of the following will be true

Accepted Answer

A)   each of the arms of the Chi structure will be a different length
B)   each of the arms will be the same length
C)   two of the arms will be the same length and the other two arms will be the same length
D)   three of the arms will be the same length and the fourth arm will be a different length
E)   none of the above are trueF) A) and E)
G) C) and D) C

Question 2

M17476PCR (Polymerase Chain Reaction)  products are of discrete length because

Accepted Answer

A)   Taq polymerase can synthesize only 1000 base pairs of DNA at a time.
B)   polymerization proceeds to the ends of the DNA.
C)   Taq polymerase is denatured after each cycle is complete.
D)   the DNA is cut with restriction enzymes prior to PCR.
E)   the reaction contains a limiting amount of nucleoside triphosphate precursors.F) D) and E)
G) A) and C)

Question 3

Segments of pure samples of identical, relatively short DNA molecules from plasmids can be subdivided into smaller pieces by

Accepted Answer

A)   Southern blot analysis.
B)   Colony hybridization
C)   Restriction enzyme digestion.
D)   Agarose gel electrophoresis.
E)   Ethidium bromide treatmentF) A) and B)
G) A) and C)

Question 4

Which of the following statements pertaining to genome organization is true?

Accepted Answer

A)   The fibroin gene undergoes DNA amplification within moth silk gland cells to enable the synthesis of up to 109 silk fibroin proteins per cell.
B)   Up to 98% of the human genome encodes proteins and structural RNA's.
C)   Histone genes are tandemly repeated and localized in nucleoli.
D)   Amplification of protein- encoding genes is commonly seen during amphibian oogenesis, when enormous stores of protein are needed.
E)   Eukaryotic chromosomes each consist of a single linear DNA molecule.F) A) and C)
G) D) and E)

Question 5

In the Sanger dideoxy method of DNA sequencing, the dideoxy analog

Accepted Answer

A)   allows specific chemical cleavage of the DNA strand.
B)   is a component of the primer.
C)   causes termination of DNA synthesis.
D)   cannot be utilized for DNA synthesis by DNA polymerase I.
E)   lacks a 5' phosphate group.F) A) and E)
G) B) and D)

Question 6

In constructing a genomic DNA library, limited digestion with a restriction enzyme that recognizes and cuts a 4 base pair sequence

Accepted Answer

A)   ensures that the DNA fragments being cloned are very small.
B)   ensures that introns are separated from exons.
C)   generates overlapping fragments of the genome.
D)   separates repetitive sequences from unique sequences.E) None of the above
F) B) and C)

Question 7

Enzymes necessary for the construction of a recombinant molecule of DNA are

Accepted Answer

A)   restriction endonucleases and DNA ligase.
B)   restriction endonucleases and Pol I.
C)   Pol I and DNA ligase.
D)   recombinase and DNA gyrase.E) All of the above
F) B) and D)

Question 8

The genomic DNA library

Accepted Answer

A)   contains DNA sequences from all species.
B)   contains plasmid DNA copies of human total RNA.
C)   contains amplified copies of a specific gene.
D)   is often constructed using phage lambda.
E)   is often screened by Southern blot hybridization.F) B) and D)
G) D) and E)

Question 9

One enzyme necessary for the genetic information of some RNA virus to be incorporated into the chromosome of a host eukaryotic cells

Accepted Answer

A)   viral polynucleotide RNA polymerase.
B)   DNA-dependent-RNA polymerase.
C)   DNA-dependent RNA ligase.
D)   RNA-dependent DNA polymerase.
E)   host cell RNA-DNA polymerase.F) B) and E)
G) C) and E)

Question 10

Chromosome walking

Accepted Answer

A)   finds overlapping cDNAs.
B)   allows long contiguous regions of DNA to be isolated.
C)   requires the synthesis of artificial chromosomes.
D)   requires somatic cell hybridization.
E)   involves the direct sequencing of genomic DNA.F) A) and E)
G) B) and E)

Question 11

All of the following are used to construct genomic libraries EXCEPT

Accepted Answer

A)   DNA ligase.
B)   bacteriophage lambda.
C)   reverse transcriptase.
D)   restriction enzymes.E) All of the above
F) A) and D)

Question 12

PCR is used for all of the following EXCEPT

Accepted Answer

A)   detection of exon deletions.
B)   amplification of a target DNA sequence.
C)   in vivo correction of a mutated gene.
D)   detection of trinucleotide repeat expansions.E) B) and C)
F) A) and B)

Question 13

Yeast artificial chromosome vectors contain all of the following EXCEPT

Accepted Answer

A)   centromeric sequences.
B)   telomeric sequences.
C)   an origin of replication.
D)   a locus control region.
E)   a selectable marker gene.F) None of the above
G) C) and E)

Question 14

DNA sequencing makes use of

Accepted Answer

A)   chemical base-modification reactions to fragment the DNA.
B)   base-specific enzymes to obtain the sequence.
C)   families of restriction enzymes of known specificity to obtain the sequence.
D)   alkaline hydrolysis and thin layer chromatography to obtain the base composition.
E)   reassociation kinetics to determine the sequence complexity of DNA.F) A) and B)
G) A) and E)

Question 15

RFLP screening during a chromosomal walk would permit the identification of a

Accepted Answer

A)   DNA interval deleted in unrelated individuals who suffer from the same genetic disease.
B)   point mutation in a gene that does not alter a restriction enzyme recognition sequence.
C)   cDNA clone for the disease of interest.
D)   clone expressing the mutant protein.E) C) and D)
F) A) and B)

Question 16

Which of the following techniques can be used to screen the general population for a single base-pair mutation in disease-causing gene?

Accepted Answer

A)   RFLP linkage analysis
B)   Allele specific oligonucleotide (ASO)  analysis
C)   Fluorescence in situ hybridization (FISH) 
D)   Multiplex PCRE) None of the above
F) C) and D) B

Question 17

According to the Holliday model, the initial steps in recombination are which of the following?

Accepted Answer

A)   Double strand breakage and rejoining of nonhomologous chromosomes
B)   Double_strand breakage and rejoining of homologous chromosomes
C)   Single-strand breakage and strand transfer between non-homologous chromosomes
D)   Single_strand breakage and strand transfer between homologous chromosomesE) C) and D)
F) A) and B)

Question 18

The "penetrance" of an inherited disease refers to the

Accepted Answer

A)   variation in phenotypes caused by differences in genetic background.
B)   proportion of individuals with a particular mutation that expresses the disease phenotype.
C)   variation in phenotypes caused by different environmental factors.
D)   tendency for the severity of the disease to increase over several generations.E) None of the above
F) A) and C)

Question 19

Which of the following would most likely be found in a recombinant plasmid containing a copy of DNA from a human gene?

Accepted Answer

A)   The entire protein coding sequence of the gene.
B)   A sequence complementary to the 3' poly-A tail of the mRNA encoded by the gene.
C)   The chromosome containing the gene.
D)   A segment of intron DNA.E) A) and D)
F) C) and D) D

Question 20

Vector development, design of the corrective gene construct, proliferation and maintenance of target cells, efficient transfection and transport of nucleic acid to the nucleus for integration into the genome, and expansion of engineered cells and implantation into the patient are all examples of

Accepted Answer

A)   challenges to successful gene therapy.
B)   techniques used to study developmental regulation.
C)   protocols used in the production of transgenic and chimeric mice.
D)   problems encountered in the development of pharmaceuticals.E) C) and D)
F) None of the above

Exam 7: Recombinant Dna and Biotechnology

Two small plasmids undergoing recombination will look like a figure 8. If they are each cut once at a unique restriction site, they will form a Chi structure. Which of the following will be true

Correct AnswerverifiedC

M17476PCR (Polymerase Chain Reaction) products are of discrete length because

Correct AnswerverifiedShow Answer

Segments of pure samples of identical, relatively short DNA molecules from plasmids can be subdivided into smaller pieces by

Correct AnswerverifiedShow Answer

Which of the following statements pertaining to genome organization is true?

Correct AnswerverifiedShow Answer

In the Sanger dideoxy method of DNA sequencing, the dideoxy analog

Correct AnswerverifiedShow Answer

In constructing a genomic DNA library, limited digestion with a restriction enzyme that recognizes and cuts a 4 base pair sequence

Correct AnswerverifiedShow Answer

Enzymes necessary for the construction of a recombinant molecule of DNA are

Correct AnswerverifiedShow Answer

The genomic DNA library

Correct AnswerverifiedShow Answer

One enzyme necessary for the genetic information of some RNA virus to be incorporated into the chromosome of a host eukaryotic cells

Correct AnswerverifiedShow Answer

Chromosome walking

Correct AnswerverifiedShow Answer

All of the following are used to construct genomic libraries EXCEPT

Correct AnswerverifiedShow Answer

PCR is used for all of the following EXCEPT

Correct AnswerverifiedShow Answer

Yeast artificial chromosome vectors contain all of the following EXCEPT

Correct AnswerverifiedShow Answer

DNA sequencing makes use of

Correct AnswerverifiedShow Answer

RFLP screening during a chromosomal walk would permit the identification of a

Correct AnswerverifiedShow Answer

Which of the following techniques can be used to screen the general population for a single base-pair mutation in disease-causing gene?

Correct AnswerverifiedB

According to the Holliday model, the initial steps in recombination are which of the following?

Correct AnswerverifiedShow Answer

The "penetrance" of an inherited disease refers to the

Correct AnswerverifiedShow Answer

Which of the following would most likely be found in a recombinant plasmid containing a copy of DNA from a human gene?

Correct AnswerverifiedD

Vector development, design of the corrective gene construct, proliferation and maintenance of target cells, efficient transfection and transport of nucleic acid to the nucleus for integration into the genome, and expansion of engineered cells and implantation into the patient are all examples of

Correct AnswerverifiedShow Answer

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C

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B

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D

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