Question 1

When an F' plasmid acts as the donor in a mating,which of the following do(es) not happen?

Accepted Answer

A)  The recipient becomes F'.
B)  The plasmid is transferred,including the chromosomal genes now on the plasmid.
C)  Some chromosomal genes not on the plasmid are transferred.
D)  All of the choices are correct.E) None of the above
F) C) and D)

Question 2

Which procedure is most associated with unsuccessful transformation and degradation of DNA?

Accepted Answer

A)  Naturally-occurring transformation with DNA fragments in competent bacteria.
B)  Induced transformation with plasmid DNA in noncompetent bacteria.
C)  Integration of DNA fragments in competent bacteria following naturally-occurring transformation.
D)  Addition of DNA fragments from competent bacteria to live,noncompetent bacteria without artificial induction.E) B) and C)
F) A) and D)

Question 3

Acridine orange is a purine base analogue.

Accepted Answer

A) True 
 B)False

Question 4

Plasmids that have genes for pili and can transfer copies of themselves to other bacteria during conjugation are called __________ plasmids.

Accepted Answer

The answer of Plasmids that have genes for pili and...

Question 5

Repair of an apurinic or apyrimidinic site in DNA by AP endonuclease must be completed by the action of

Accepted Answer

A)  UvrABC endonuclease.
B)  RecA protein.
C)  DNA polymerase I.
D)  None of the choices are correct.E) C) and D)
F) B) and D)

Question 6

Which of the following is not true of specialized transduction?

Accepted Answer

A)  It is carried out by temperate phage.
B)  It is restricted to those genes on either side of an integrated prophage.
C)  The phage genome retains the full complement of phage genes.
D)  All of the choices are correct.E) B) and C)
F) A) and C)

Question 7

If you wished to study the SOS response in Escherichia coli,which of the following experimental procedures could be used?

Accepted Answer

A)  All of the choices are correct.
B)  Expose cells to nonionizing radiation for 20 minutes.
C)  Expose cells to ionizing radiation for 10 minutes.
D)  Transfer log phase cells from complete medium to thymine minimal medium.
E)  Inactivate genes that encode DNA polymerases.F) A) and B)
G) B) and C)

Question 8

When bacterial genes are transferred to another bacterium by a virus,it is called

Accepted Answer

A)  conjugation.
B)  transformation.
C)  transduction.
D)  transfection.E) A) and D)
F) A) and B)

Question 9

Which type of medium support growth of mutant bacteria that are threonine auxotrophs?

Accepted Answer

A)  Threonine auxotrophs only grow on media that is supplemented with threonine.
B)  Threonine auxotrophs only grow on media that lacks threonine.
C)  Since threonine is essential for growth,threonine auxotrophs do not grow on any type of media.
D)  Since threonine is essential for growth,threonine auxotrophs can grow on any type of media.E) A) and D)
F) A) and C)

Question 10

SOS repair

Accepted Answer

A)  requires RecA protein.
B)  is inducible by DNA damage.
C)  is error prone,i.e.,produces mutations.
D)  All of the choices are correct.E) A) and B)
F) B) and C)

Question 11

What would be the hypothetical outcome if the action of RecA were inhibited during the SOS response?

Accepted Answer

A)  LexA would not autolyse,and therefore the transcription of DNA repair genes would not occur.
B)  LexA would autolyse,and therefore the transcription of DNA repair genes would occur.
C)  LexA would autolyse,and therefore the transcription of repair genes would not occur.
D)  LexA would not autolyse,and therefore the transcription of repair genes would occur.E) B) and C)
F) All of the above

Question 12

Which of the following methods of genetic recombination is common to both eukaryotic and bacterial cells?

Accepted Answer

A)  Vertical gene transfer
B)  Crossing over
C)  Gamete fusion
D)  All of the choices are correct.E) None of the above
F) All of the above

Question 13

Transposons that insert at a new location while a copy remains at the original location are said to mediate _______________ transposition.

Accepted Answer

A)  simple
B)  composite
C)  incomplete
D)  replicativeE) A) and D)
F) A) and B)

Question 14

Which statement best summarizes the major distinction between horizontal gene transfer and vertical gene transfer?

Accepted Answer

A)  The recipient of genes in horizontal transfer is a mature cell,whereas the recipient of genes in vertical transfer is a progeny cell.
B)  Horizontal transfer is observed in bacteria and archaea,whereas vertical transfer is observed in all cells.
C)  Organisms that use horizontal transfer can only reproduce asexually,whereas organisms that use vertical transfer can reproduce sexually or asexually.
D)  Genes are more stable in recipient cells following horizontal transfer that in those following vertical transfer.E) A) and D)
F) None of the above

Question 15

After an F+ * F- mating,the donor is __________ and the recipient is __________.

Accepted Answer

A)  F+; F-
B)  F-; F+
C)  F-; F-
D)  F+; F+E) B) and D)
F) A) and C)

Question 16

The genome of a recipient cell is called a(n) __________.

Accepted Answer

A)  merozygote
B)  exogenote
C)  endogenote
D)  None of the choices are correct.E) A) and D)
F) B) and C)

Question 17

All of the following are appropriate strategies for overcoming drug resistance in bacteria except

Accepted Answer

A)  decreasing the use of antibiotics in agriculture.
B)  patient compliance with dosage instructions when taking antibiotics.
C)  heightened infection control in health care facilities.
D)  prudent use of antibiotics when treating viral infections.E) None of the above
F) All of the above

Question 18

Conjugative transposons

Accepted Answer

A)  can be transferred from one bacterium to another by a phage mediated process.
B)  may be involved in the spread of antibiotic resistance in bacteria.
C)  often express enzymes that degrade aromatic compounds.
D)  All of the choices are correct.E) A) and D)
F) B) and C)

Question 19

Lethal mutations can be recovered in haploid organisms if they are

Accepted Answer

A)  dominant.
B)  active.
C)  conditional.
D)  None of the choices are correct.E) A) and D)
F) A) and B)

Question 20

Which of the following effects may be mediated by transposable elements?

Accepted Answer

A)  A mutation may result from insertion into a gene.
B)  Activation of nearby genes may occur.
C)  Genetic deletions may form.
D)  All of the choices are correct.E) None of the above
F) A) and D)

Exam 16: Mechanisms of Genetic Variation

Conjugative transposons

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When bacterial genes are transferred to another bacterium by a virus,it is called

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All of the following are appropriate strategies for overcoming drug resistance in bacteria except

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Lethal mutations can be recovered in haploid organisms if they are

Correct AnswerverifiedShow Answer

If you wished to study the SOS response in Escherichia coli,which of the following experimental procedures could be used?

Correct AnswerverifiedShow Answer

Which of the following methods of genetic recombination is common to both eukaryotic and bacterial cells?

Correct AnswerverifiedShow Answer

When an F' plasmid acts as the donor in a mating,which of the following do(es) not happen?

Correct AnswerverifiedShow Answer

Which statement best summarizes the major distinction between horizontal gene transfer and vertical gene transfer?

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Plasmids that have genes for pili and can transfer copies of themselves to other bacteria during conjugation are called __________ plasmids.

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SOS repair

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Which of the following is not true of specialized transduction?

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What would be the hypothetical outcome if the action of RecA were inhibited during the SOS response?

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Acridine orange is a purine base analogue.

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The genome of a recipient cell is called a(n) __________.

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After an F+ * F- mating,the donor is __________ and the recipient is __________.

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Which procedure is most associated with unsuccessful transformation and degradation of DNA?

Correct AnswerverifiedShow Answer

Repair of an apurinic or apyrimidinic site in DNA by AP endonuclease must be completed by the action of

Correct AnswerverifiedShow Answer

Transposons that insert at a new location while a copy remains at the original location are said to mediate _______________ transposition.

Correct AnswerverifiedShow Answer

Which of the following effects may be mediated by transposable elements?

Correct AnswerverifiedShow Answer

Which type of medium support growth of mutant bacteria that are threonine auxotrophs?

Correct AnswerverifiedShow Answer

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After an F⁺ * F^- mating,the donor is and the recipient is .

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