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Exam 6: Microbial Growth

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Question 31

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Assume you inoculated 100 cells,with a generation time of 20 minutes,into 100 ml of nutrient broth.You then inoculated 100 cells of the same species into 200 ml of nutrient broth.After incubation for 3 hours,you can reasonably expect to have

A) more cells in the 100 ml.
B) more cells in the 200 ml.
C) the same number of cells in both.
D) The answer cannot be determined based on the information provided.

E) C) and D)
F) B) and C)

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Question 32

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An isolated colony on a streak plate contains millions (or even billions)of identical cells all arising from one initial cell.

A) True
B) False

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Question 33

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Table 6.1 Three different culture media are shown below. $\begin{array} { | c | c | c | } \hline \text { Medium A } & \text { Medium B } & \text { Medium C } \\\hline \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } & \text { Yeast extract } & \text { Glucose } \\\hline \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \text { Beef extract } & \text { Peptone } \\\hline \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \left( \mathrm { NH } _ { 4 } \right) _ { 2 } \mathrm { SO } _ { 4 } \\\hline \mathrm { CaC } _ { 2 } & \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 2 } \\\hline \mathrm { NaHCO } _ { 3 } & ( \mathrm { NH } 4 ) _ { 2 } \mathrm { SO } _ { 4 } & \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } \\\hline\end{array}$ -For the three types of media in Table 6.1,which medium (or media) is/are chemically defined?

Most fungi grow best at pH

Gelatin is used as a solidifying agent in microbiological media since few bacteria can degrade it.

Table 6.1 Three different culture media are shown below. $\begin{array} { | c | c | c | } \hline \text { Medium A } & \text { Medium B } & \text { Medium C } \\\hline \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } & \text { Yeast extract } & \text { Glucose } \\\hline \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \text { Beef extract } & \text { Peptone } \\\hline \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \left( \mathrm { NH } _ { 4 } \right) _ { 2 } \mathrm { SO } _ { 4 } \\\hline \mathrm { CaC } _ { 2 } & \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 2 } \\\hline \mathrm { NaHCO } _ { 3 } & ( \mathrm { NH } 4 ) _ { 2 } \mathrm { SO } _ { 4 } & \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } \\\hline\end{array}$ -In Table,6.1,in which medium (or media) would an autotroph grow but a heterotroph could not?

Which enzyme catalyzes the reaction: O₂- + O₂- + 2H⁺ → H₂O₂ + O₂?

During which growth phase will gram-positive bacteria be most susceptible to penicillin?

You are tasked with attempting to culture microbes in the lab that were initially obtained from samples taken in and near the Arctic circle,in areas of extreme cold and either very long or very short days,depending on the time of year.How would you begin to try to grow these microbes in the lab,and why would you choose those methods?

Which of the following is an example of a metabolic activity that could be used to measure microbial growth?

Which enzyme catalyzes the following reaction? 2H₂O2 → 2H₂O + O₂

Table 6.2 The following data show growth of two bacteria on different media. The data in Table 6.2 indicate that S.aureus is a(n)

The biosafety level (BSL) for a clinical microbiology laboratory working with potentially airborne pathogens,such as tuberculosis bacteria,is

Bacterial growth refers to an increase in the numbers of cells in a bacterial culture.

Figure 6.2 -Figure 6.2 shows a typical bacterial growth curve with the y-axis indicating the log of the number of bacteria and the x-axis indicating time in culture.In the figure,which sections of the graph illustrate a logarithmic change in cell numbers?

An extreme-weather event has knocked out power in your community.Even with rebuilding efforts,it appears that it could be 6 months or longer before power is restored.Since you have a background in microbiology,the people you know are asking you for possible ways of storing/preserving their food safely without electricity.What advice can you give them,and why would you make those recommendations?

Thirty-six colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard plate count.How many viable and culturable cells were in the original sample?

Most bacteria grow best at pH

The term aerotolerant anaerobe refers to an organism that

The biosafety level (BSL) for most introductory microbiology laboratories is