Question 1

The reaction catalyzed by DNA polymerase.

Accepted Answer

A)   mRNA -cDNA
B)   DNA -mRNA
C)   mRNA -protein
D)   DNA -DNA
E)   NoneF) B) and E)
G) C) and D)

Question 2

A source of heat- stable DNA polymerase is

Accepted Answer

A)   Pseudomonas.
B)   Saccharomyces cerevisiae.
C)   Bacillus thuringiensis.
D)   Thermus aquaticus.
E)   Agrobacterium tumefaciens.F) A) and E)
G) C) and D)

Question 3

The following steps must be performed to make a bacterium produce human protein X: 1- Translation; 2- Restriction enzyme; 3- Prokaryotic transcription; 4- DNA ligase;
5- Transformation; 6- Eukaryotic transcription; 7- Reverse transcription. Put the steps in the correct sequence.

Accepted Answer

A)   1, 2, 3, 5, 4, 7, 6
B)   6, 7, 2, 3, 4, 5, 1
C)   6, 2, 1, 3, 4, 5, 7
D)   5, 2, 3, 4, 7, 6, 1
E)   6, 7, 2, 4, 5, 3, 1F) None of the above
G) A) and C)

Question 4

You have a small gene that you wish replicated by PCR. You add radioactively labeled nucleotides to the PCR thermocycler. After 3 replication cycles, what percentage of the DNA single- strands are radioactively labeled?

Accepted Answer

A)   87.5%
B)   0%
C)   50%
D)   12.5%
E)   100%F) C) and E)
G) All of the above

Question 5

Which of the following is not an agricultural product made by DNA techniques?

Accepted Answer

A)   Bacillus thuringiensis insecticide
B)   Nitrogenase (nitrogen fixation) 
C)   Glyphosate resistance
D)   Drought resistance
E)   Frost retardantF) All of the above
G) B) and C)

Question 6

Figure 9.1
 
-In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the ampicillin- resistance (amp)  gene?

Accepted Answer

A)   0.17 kilobase pairs
B)   3.00 kbp
C)   0.25 kbp
D)   1.50 kbp
E)   1.08 kbpF) B) and C)
G) A) and E)

Question 7

Figure 9.3
 
-In Figure 9.3, the purpose of this experiment is to

Accepted Answer

A)   Put a gene in Bacillus.
B)   Put a gene into a plant.
C)   Put an insecticide on plant leaves.
D)   Isolate Pseudomonas from a plant.
E)   None of the above.F) D) and E)
G) C) and E)

Question 8

PCR can be used to identify an unknown bacterium because

Accepted Answer

A)   DNA can be electrophoresed.
B)   All cells have DNA.
C)   DNA polymerase will replicate DNA.
D)   The RNA primer is specific.
E)   None of the above.F) All of the above
G) B) and E)

Question 9

PCR can be used to amplify DNA in a clinical sample. The following steps are used in PCR. What is the fourth step?

Accepted Answer

A)   Incubate at 72°C.
B)   Collect DNA.
C)   Incubate at 94°C.
D)   Incubate at 60°C.
E)   Add DNA polymerase.F) B) and C)
G) D) and E)

Question 10

If you have inserted a gene in the Ti, the next step in genetic engineering is

Accepted Answer

A)   Splicing Ti into a plasmid.
B)   Transformation of E. coli with Ti.
C)   Transformation of an animal cell.
D)   Inserting Ti into Agrobacterium.
E)   None of the above.F) A) and C)
G) C) and D)

Question 11

Which of the following is not involved in making cDNA?

Accepted Answer

A)   Transcription.
B)   RNA processing to remove introns.
C)   Reverse transcription.
D)   Translation.E) A) and B)
F) B) and C)

Question 12

Foreign DNA can be inserted into cells by all of the following except

Accepted Answer

A)   A gene gun.
B)   Electroporation.
C)   Transformation.
D)   Protoplast fusion.
E)   None of the above.F) A) and B)
G) C) and E)

Question 13

Suicide genes can be controlled by the fimbriae- gene operator. This would result in the death of

Accepted Answer

A)   Cells at 37°C.
B)   All cells.
C)   Cells making fimbriae.
D)   Conjugating cells.
E)   Cells making flagella.F) A) and C)
G) B) and E)

Question 14

Which organism naturally possesses the Ti plasmid?

Accepted Answer

A)   Pseudomonas
B)   Saccharomyces cervisiae
C)   Bacillus thuringiensis
D)   Thermus aquaticus
E)   Agrobacterium tumefaciensF) A) and D)
G) A) and C)

Question 15

Figure 9.3
 
-In Figure 9.3, the resulting organism (a)  is

Accepted Answer

A)   Pseudomonas fluorescens.
B)   a plant × Pseudomonas hybrid.
C)   Bacillus thuringiensis.
D)   E. coli.
E)   A tomato plant.F) B) and D)
G) A) and E)

Question 16

Which organism degrades PCBs and has been engineered to produce BT toxin?

Accepted Answer

A)   Pseudomonas
B)   Bacillus thuringiensis
C)   Saccharomyces cerevisiae
D)   Thermus aquaticus
E)   Agrobacterium tumefaciensF) All of the above
G) C) and E)

Question 17

Which of the following can be used to make recombinant DNA?

Accepted Answer

A)   Protoplast fusion
B)   Tungsten "bullets"
C)   Transformation
D)   Microinjection
E)   All of the above.F) C) and D)
G) A) and D)

Question 18

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is

Accepted Answer

A)   Determine the nucleotide sequence for the improved enzyme.
B)   Synthesize the gene for the improved enzyme.
C)   Mutate bacteria until one makes the improved enzyme.
D)   Look for a bacterium that makes the improved enzyme.
E)   None; the enzyme can't be produced.F) C) and D)
G) A) and E)

Question 19

The use of an antibiotic resistance gene on a plasmid used in genetic engineering makes

Accepted Answer

A)   The recombinant cell unable to survive.
B)   Replica plating possible.
C)   Direct selection possible.
D)   The recombinant cell dangerous.
E)   All of the above.F) A) and B)
G) B) and E)

Question 20

Large numbers of bacterial cells are not found in crown galls because

Accepted Answer

A)   The assumption is not true; many bacteria are in the galls.
B)   A gene in plant cells is controlling growth.
C)   The plant kills the bacteria.
D)   Cell walls protect the plant from bacterial invasion.
E)   None of the above.F) B) and C)
G) A) and C)

Exam 9: Biotechnology and Recombinant DNA

A source of heat- stable DNA polymerase is

Correct AnswerverifiedShow Answer

Which of the following is not an agricultural product made by DNA techniques?

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A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is

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If you have inserted a gene in the Ti, the next step in genetic engineering is

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Figure 9.3 -In Figure 9.3, the resulting organism (a) is

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Which organism naturally possesses the Ti plasmid?

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Which organism degrades PCBs and has been engineered to produce BT toxin?

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Which of the following can be used to make recombinant DNA?

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PCR can be used to identify an unknown bacterium because

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Large numbers of bacterial cells are not found in crown galls because

Correct AnswerverifiedShow Answer

The following steps must be performed to make a bacterium produce human protein X: 1- Translation; 2- Restriction enzyme; 3- Prokaryotic transcription; 4- DNA ligase; 5- Transformation; 6- Eukaryotic transcription; 7- Reverse transcription. Put the steps in the correct sequence.

Correct AnswerverifiedShow Answer

Figure 9.3 -In Figure 9.3, the purpose of this experiment is to

Correct AnswerverifiedShow Answer

Which of the following is not involved in making cDNA?

Correct AnswerverifiedShow Answer

The use of an antibiotic resistance gene on a plasmid used in genetic engineering makes

Correct AnswerverifiedShow Answer

PCR can be used to amplify DNA in a clinical sample. The following steps are used in PCR. What is the fourth step?

Correct AnswerverifiedShow Answer

Foreign DNA can be inserted into cells by all of the following except

Correct AnswerverifiedShow Answer

You have a small gene that you wish replicated by PCR. You add radioactively labeled nucleotides to the PCR thermocycler. After 3 replication cycles, what percentage of the DNA single- strands are radioactively labeled?

Correct AnswerverifiedShow Answer

Suicide genes can be controlled by the fimbriae- gene operator. This would result in the death of

Correct AnswerverifiedShow Answer

The reaction catalyzed by DNA polymerase.

Correct AnswerverifiedShow Answer

Figure 9.1 -In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the ampicillin- resistance (amp) gene?

Correct AnswerverifiedShow Answer

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