Question 1

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

Accepted Answer

A)   circular form of DNA or integrates into the host chromosome
B)   may replicate in several species
C)   self-replication
D)   large size
E)   has a selectable markerF) C) and D)
G) A) and E)

Question 2

An advantage of cDNA over genomic DNA is that it

Accepted Answer

A)   lacks exons.
B)   is very easy to isolate.
C)   contains selectable markers.
D)   lacks introns.
E)   can form very large DNA segments.F) B) and D)
G) A) and C)

Question 3

Biotechnology involves the

Accepted Answer

A)   use of animal cells to make vaccines.
B)   use of microorganisms to make desired products.
C)   development of disease-resistant crop plants.
D)   use of microorganisms to make desired products, the use of animal cells to make vaccines, and the development of disease-resistant crop plants.
E)   use of microorganisms to make desired products and the use of animal cells to make vaccines.F) A) and D)
G) A) and E)

Question 4

A restriction fragment is

Accepted Answer

A)   a segment of mRNA.
B)   a gene.
C)   cDNA.
D)   a segment of DNA.
E)   a segment of tRNA.F) A) and C)
G) A) and B)

Question 5

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes

Accepted Answer

A)   direct selection possible.
B)   the recombinant cell unable to survive.
C)   the recombinant cell dangerous.
D)   replica plating possible.
E)   All of the answers are correct.F) B) and C)
G) None of the above

Question 6

The study of genetic material taken directly from the environment is

Accepted Answer

A)   reverse genetics.
B)   proteomics.
C)   bioinformatics.
D)   forensic microbiology.
E)   metagenomics.F) None of the above
G) B) and C)

Question 7

The following steps must be performed to make a bacterium produce human protein X. 1- Translation
2- Restriction enzyme
3- Prokaryotic transcription 4-DNA ligase
5- Transformation
6- Eukaryotic transcription 7-Reverse transcription
Which of the following places the steps in the correct order?

Accepted Answer

A)   6, 7, 2, 3, 4, 5, 1
B)   6, 2, 1, 3, 4, 5, 7
C)   1, 2, 3, 5, 4, 7, 6
D)   6, 7, 2, 4, 5, 3, 1
E)   5, 2, 3, 4, 7, 6, 1F) A) and B)
G) All of the above D

Question 8

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Accepted Answer

A)   transformation of an animal cell.
B)   inserting the Ti plasmid into a plant cell.
C)   inserting the Ti plasmid into Agrobacterium.
D)   splicing T DNA into a plasmid.
E)   transformation of E. coli with Ti plasmid.F) D) and E)
G) C) and D)

Question 9

Restriction enzymes are

Accepted Answer

A)   bacterial enzymes that splice DNA.
B)   animal enzymes that splice RNA.
C)   bacterial enzymes that destroy phage DNA.
D)   viral enzymes that destroy host DNA.E) A) and B)
F) A) and C)

Question 10

The use of ʺsuicideʺ genes in genetically modified organisms is designed to

Accepted Answer

A)   provide a means to eliminate non-modified organisms.
B)   prevent the growth of the modified organisms in the environment.
C)   provide for resistance of the modified organisms to pesticides.
D)   delete genes necessary for modified organismʹs growth.
E)   kill the modified organisms before they are released in the environment.F) A) and B)
G) A) and C)

Question 11

The random shotgun method is used in

Accepted Answer

A)   amplification of unknown DNA.
B)   genome sequencing.
C)   transforming plant cells with recombinant DNA.
D)   forensic microbiology.
E)   RFLP analysis.F) A) and B)
G) A) and C)

Question 12

The disadvantage of genomic libraries over cDNA libraries is that genomic libraries contain gene introns.

Accepted Answer

A) True 
 B)False

Question 13

The local public health agency has received reports of an outbreak of Salmonella gastroenteritis among attendees at a city-sponsored chili cook-off. What techniques from recombinant DNA technology would the agency likely use to investigate this outbreak? Describe the expected results from these techniques.

Accepted Answer

The local public health agency would lik...

Question 14

PCR can be used to identify an unknown bacterium because

Accepted Answer

A)   all cells have RNA.
B)   DNA polymerase will replicate DNA.
C)   all cells have DNA.
D)   DNA can be electrophoresed.
E)   the RNA primer is specific.F) C) and E)
G) A) and E)

Question 15

A population of cells carrying a desired plasmid is called a

Accepted Answer

A)   clone.
B)   vector.
C)   Southern blot.
D)   library.
E)   PCR.F) B) and C)
G) A) and B) A

Question 16

The Pap test for cervical cancer involves microscopic examination of cervical cells for cancerous cells. A new, rapid diagnostic test to detect human papilloma virus (HPV)  DNA before cancer develops is done without microscopic exam. The steps involved in this FastHPV test are listed below. What is the second step?

Accepted Answer

A)   Add an RNA probe for HPV DNA.
B)   Add enzyme substrate.
C)   The order is unimportant.
D)   Add enzyme-linked antibodies against DNA-RNA.
E)   Lyse human cells.F) A) and B)
G) None of the above

Question 17

Figure 9.5
 
-In Figure 9.5, the gene that allows the plasmid to be self-replicating is

Accepted Answer

A)   lacZ.
B)   ori.
C)   HindIII.
D)   ampR.
E)   EcoRI.F) All of the above
G) A) and D)

Question 18

The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction.

Accepted Answer

A) True 
 B)False  True

Question 19

Nearly all cells, including E. coli and yeast, naturally take up DNA from their surroundings without chemical treatment.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following places the steps in the PCR procedure in the correct order?
1)  Incubate at 94°C to denature DNA strands;
2)  Incubate at 72°C for DNA synthesis;
3)  Incubate at 60°C for primer hybridization.

Accepted Answer

A)   2; 1; 3
B)   1, 2, 3
C)   3, 2, 1
D)   1, 3, 2
E)   3; 1; 2F) B) and E)
G) B) and D)

Exam 9: Biotechnology and Dna Technology

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

Correct Answer
verified

An advantage of cDNA over genomic DNA is that it

Correct Answer
verified

Biotechnology involves the

Correct Answer
verified

A restriction fragment is

Correct Answer
verified

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes

Correct Answer
verified

The study of genetic material taken directly from the environment is

Correct Answer
verified

The following steps must be performed to make a bacterium produce human protein X. 1- Translation 2- Restriction enzyme 3- Prokaryotic transcription 4-DNA ligase 5- Transformation 6- Eukaryotic transcription 7-Reverse transcription Which of the following places the steps in the correct order?

Correct Answer
verified
D

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Correct Answer
verified

Restriction enzymes are

Correct Answer
verified

The use of ʺsuicideʺ genes in genetically modified organisms is designed to

Correct Answer
verified

The random shotgun method is used in

Correct Answer
verified

The disadvantage of genomic libraries over cDNA libraries is that genomic libraries contain gene introns.

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
The local public health agency would lik...
View Answer

PCR can be used to identify an unknown bacterium because

Correct Answer
verified

A population of cells carrying a desired plasmid is called a

Correct Answer
verified
A

Correct Answer
verified

Figure 9.5 -In Figure 9.5, the gene that allows the plasmid to be self-replicating is

Correct Answer
verified

The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction.

Correct Answer
verified
True

Nearly all cells, including E. coli and yeast, naturally take up DNA from their surroundings without chemical treatment.

Correct Answer
verified

Which of the following places the steps in the PCR procedure in the correct order? 1) Incubate at 94°C to denature DNA strands; 2) Incubate at 72°C for DNA synthesis; 3) Incubate at 60°C for primer hybridization.

Correct Answer
verified

Exam 9: Biotechnology and Dna Technology

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

Correct AnswerverifiedShow Answer

An advantage of cDNA over genomic DNA is that it

Correct AnswerverifiedShow Answer

Biotechnology involves the

Correct AnswerverifiedShow Answer

A restriction fragment is

Correct AnswerverifiedShow Answer

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes

Correct AnswerverifiedShow Answer

The study of genetic material taken directly from the environment is

Correct AnswerverifiedShow Answer

The following steps must be performed to make a bacterium produce human protein X. 1- Translation 2- Restriction enzyme 3- Prokaryotic transcription 4-DNA ligase 5- Transformation 6- Eukaryotic transcription 7-Reverse transcription Which of the following places the steps in the correct order?

Correct AnswerverifiedD

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Correct AnswerverifiedShow Answer

Restriction enzymes are

Correct AnswerverifiedShow Answer

The use of ʺsuicideʺ genes in genetically modified organisms is designed to

Correct AnswerverifiedShow Answer

The random shotgun method is used in

Correct AnswerverifiedShow Answer

The disadvantage of genomic libraries over cDNA libraries is that genomic libraries contain gene introns.

Correct AnswerverifiedShow Answer

Correct AnswerAnswered by ExamLex AIThe local public health agency would lik...View AnswerShow Answer

PCR can be used to identify an unknown bacterium because

Correct AnswerverifiedShow Answer

A population of cells carrying a desired plasmid is called a

Correct AnswerverifiedA

Correct AnswerverifiedShow Answer

Figure 9.5 -In Figure 9.5, the gene that allows the plasmid to be self-replicating is

Correct AnswerverifiedShow Answer

The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction.

Correct AnswerverifiedTrue

Nearly all cells, including E. coli and yeast, naturally take up DNA from their surroundings without chemical treatment.

Correct AnswerverifiedShow Answer

Which of the following places the steps in the PCR procedure in the correct order? 1) Incubate at 94°C to denature DNA strands; 2) Incubate at 72°C for DNA synthesis; 3) Incubate at 60°C for primer hybridization.

Correct AnswerverifiedShow Answer

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D

Correct Answer
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verified

Correct Answer
Answered by ExamLex AI
The local public health agency would lik...
View Answer

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verified

Correct Answer
verified
A

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verified

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verified

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verified
True

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